APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
FGF4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4.The immunogen is located within the last 50 amino acids of FGF4.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibroblast growth factor 4 , FGF-4 , HBGF-4 , HST , HST-1 , HSTF-1 , HSTF1 , K-FGF , KFGF
Cellular Localization
Secreted
Background
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
Database
Research Area
DATA IMAGES
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GTX85040 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX85040 FGF4 antibody.
Working concentration : 2.5 μg/ml
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GTX85040 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX85040 FGF4 antibody.
Working concentration : 2.5 μg/ml
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GTX85040 WB Image
WB analysis of NIH 3T3 cell lysate using GTX85040 FGF4 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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REFERENCE
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REVIEW
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