APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
4μg/ml |
Note :
IHC-P
Human Small Intestine shows staining of the enterocytic cytoplasm.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89588-PEP).
Product Note
This antibody is expected to recognise five isoforms (as represented by NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CLPRHPAQLANGGLKR, from the C Terminus of the protein sequence according to NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibroblast growth factor receptor 1 , BFGFR , CD331 , CEK , ECCL , FGFBR , FGFR-1 , FLG , FLT-2 , FLT2 , HBGFR , HH2 , HRTFDS , KAL2 , N-SAM , OGD , bFGF-R-1
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Nucleus , Cytoplasm, cytosol , Cytoplasmic vesicle
Background
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89588 IHC-P Image
IHC-P analysis of human small intestine using GTX89588 FGFR1 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 4μg/ml
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GTX89588 WB Image
WB analysis of human breast lysate using GTX89588 FGFR1 antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX89588 IHC-P Image
IHC-P analysis of human kidney using GTX89588 FGFR1 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 4μg/ml
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REFERENCE
There are currently no references for FGFR1 antibody, C-term (GTX89588). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FGFR1 antibody, C-term (GTX89588). Be the first to share your experience with this product.