Untreated (–) and treated (+) A549 whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr766) antibody (GTX133459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.