GeneTex
United States (US)

FGFR1 (phospho Tyr766) antibody

GTX32182_20190131_IHC-P_19013102_813.jpg
GTX32182_20190131_WB_19013102_349.jpg

Cat No. GTX32182

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

92 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of FGFR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibroblast Growth Factor Receptor 1,Bfgfr,Cd331,Cek,Eccl,Fgfbr,Fgfr-1,Flg,Flt-2,Flt2,Hbgfr,Hh2,Hrtfds,Kal2,N-Sam,Ogd,Bfgf-R-1,Fgfr1

Cellular Localization

Cell membrane,Cytoplasm,Cytoplasmic vesicle,Cytosol,Nucleus

Background

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32182_20190131_IHC-P_19013102_813.jpg

GTX32182 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32182 FGFR1 (phospho Tyr766) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32182_20190131_WB_19013102_349.jpg

GTX32182 WB Image

WB analysis of EGF-treated HepG2 (A), EGF-treated SP2/0 (B), EGF-treated PC12 (C) whole cell lysates using GTX32182 FGFR1 (phospho Tyr766) antibody.

Package List Price ($)
$ 319