*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88618-PEP).
This antibody is expected to recognise all reported isoforms except isoform 7 (NP_001138389.1). The immunizing peptide represents part of the extracellular domain.
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-GREKEITASPDY, from the internal region of the protein sequence according to NP_000132.3; NP_075259.4; NP_001138385.1; NP_001138386.1; NP_001138387.1; NP_001138388.1; NP_001138390.1; NP_001138391.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Fibroblast Growth Factor Receptor 2,Bbds,Bek,Bfr-1,Cd332,Cek3,Cfd1,Ect1,Jws,K-Sam,Kgfr,Tk14,Tk25,Fgfr2
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
GTX88618 WB Image
WB analysis of A549 lysate using GTX88618 FGFR2 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer