GeneTex

  • Country / Location Selection

United States (US)

FGFR2 antibody

Anti-FGFR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25476
Anti-FGFR2 antibody used in Western Blot (WB). GTX25476
Anti-FGFR2 antibody used in Flow cytometry (FACS). GTX25476
Anti-FGFR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX25476
Anti-FGFR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX25476
Anti-FGFR2 antibody used in Flow cytometry (FACS). GTX25476

Cat. No. GTX25476

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all FGFR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50-1:100
IHC-P 1:50-1:100
FACS 1:25
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Jurkat cell lysate.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FGFR2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_380552

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast growth factor receptor 2 , BBDS , BEK , BFR-1 , CD332 , CEK3 , CFD1 , ECT1 , JWS , K-SAM , KGFR , TK14 , TK25

Cellular Localization

Cell membrane; Single-pass type I membrane protein,Golgi apparatus,Cytoplasmic vesicle

Background

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-FGFR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25476

GTX25476 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25476 FGFR2 antibody.
Green : FGFR
Red : Actin filament
Dilution : 1:25

Anti-FGFR2 antibody used in Western Blot (WB). GTX25476

GTX25476 WB Image

WB analysis of various samples using GTX25476 FGFR2 antibody.
Lane 1: DU145 whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: T47D whole cell lysate
Lane 4: HeLa whole cell lysate
Lane 5: K562 whole cell lysate
Lane 6: M.brain whole lysate
Loading : 20 μg per lane
Dilution : 1:1000-1:2000

Anti-FGFR2 antibody used in Flow cytometry (FACS). GTX25476

GTX25476 FACS Image

FACS analysis of HeLa cells using GTX25476 FGFR2 antibody.
Green : FGFR2
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-FGFR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX25476

GTX25476 IHC-P Image

IHC-P analysis of human liver tissue using GTX25476 FGFR2 antibody.
Dilution : 1:25

Anti-FGFR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX25476

GTX25476 IHC-P Image

IHC-P analysis of human brain tissue using GTX25476 FGFR2 antibody.
Dilution : 1:25

Anti-FGFR2 antibody used in Flow cytometry (FACS). GTX25476

GTX25476 FACS Image

FACS analysis of HeLa cells using GTX25476 FGFR2 antibody.
Green : FGFR2
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

REFERENCE

Application Reference

Kawane T et al. Sci Rep 2018; 8 (1) : 13551 Runx2 is required for the proliferation of osteoblast progenitors and induces proliferation by regulating Fgfr2 and Fgfr3.
Application : IHC-P
Reactivity : Mouse
Hu Y et al. Carcinogenesis 2014; 35 (7) : 1491-9 The FGFR/MEK/ERK/brachyury pathway is critical for chordoma cell growth and survival.
Application : WB
Reactivity : Human
French DM et al. PLoS One 2012; 7 (5) : e36713 Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for FGFR2 antibody (GTX25476). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399