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FGFR4 antibody

Comparison
Anti-FGFR4 antibody used in Western Blot (WB). GTX134355
Anti-FGFR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX134355
Anti-FGFR4 antibody used in Western Blot (WB). GTX134355

Cat. No. GTX134355

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FGFR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human FGFR4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887257

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast growth factor receptor 4 , CD334 , JTK2 , TKF

Cellular Localization

Cell membrane; Single-pass type I membrane protein,Endosome,Endoplasmic reticulum

Background

The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-FGFR4 antibody used in Western Blot (WB). GTX134355

GTX134355 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR4 antibody (GTX134355) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FGFR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX134355

GTX134355 IHC-P Image

FGFR4 antibody detects FGFR4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
FGFR4 stained by FGFR4 antibody (GTX134355) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FGFR4 antibody used in Western Blot (WB). GTX134355

GTX134355 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with FGFR4 antibody (GTX134355) diluted at 1:500 and competitor's antibody (#2894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Pal SC et al. Genes (Basel) 2023; 14 (10) Molecular Mechanisms Involved in MAFLD in Cholecystectomized Patients: A Cohort Study.
SC Pal et al. Genes 2023; Molecular Mechanisms Involved in MAFLD in Cholecystectomized Patients: A Cohort Study
Application : IHC-P
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169