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FGFR5 antibody

Anti-FGFR5 antibody used in Western Blot (WB). GTX103778
Anti-FGFR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103778
Anti-FGFR5 antibody used in Western Blot (WB). GTX103778

Cat No. GTX103778

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2 , HepG2 membrane extract , FGFRL1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FGFR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibroblast growth factor receptor like 1 , FGFR5 , FHFR

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-FGFR5 antibody used in Western Blot (WB). GTX103778

GTX103778 WB Image

HepG2 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FGFR5 antibody (GTX103778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FGFR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103778

GTX103778 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Oral CA Cal27 xenograft, using FGFRL1(GTX103778) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FGFR5 antibody used in Western Blot (WB). GTX103778

GTX103778 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FGFR5 antibody (GTX103778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for FGFR5 antibody (GTX103778). Be the first to share your publications with this product.
REVIEW

FGFR5 antibody Cat No. GTX103778

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 09-Nov-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF-7 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169