APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.05-0.3μg/ml |
| 2μg/ml |
|
Note :
IHC-P
Human Testis shows fibrous staining in the cytoplasm of developing sperm cells..
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89663-PEP).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.2; NP_001153171.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
four and a half LIM domains 1 , FCMSU , FHL-1 , FHL1A , FHL1B , FLH1A , KYOT , RBMX1A , RBMX1B , SLIM , SLIM-1 , SLIM1 , SLIMMER , XMPMA
Cellular Localization
Nucleus , Cytoplasm, cytosol
Background
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
|
GTX89663 IHC-P Image
IHC-P analysis of human testis using GTX89663 FHL1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
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GTX89663 WB Image
WB analysis of human muscle lysate using GTX89663 FHL1 antibody, Internal.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for FHL1 antibody, Internal (GTX89663). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FHL1 antibody, Internal (GTX89663). Be the first to share your experience with this product.
