GeneTex
United States (US)

FHL1 antibody

GTX100266_WB_19011506_225.jpg
GTX100266_39568_20160317_WB_M_Merged_19011506_409.jpg

Cat No. GTX100266

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa , mouse muscle , mouse lung

Specificity/Sensitivity

Based on sequence homology, GTX100266 is expected to recognize isoform 1 (FHL1A), isoform 4, and isoform 5, but not isoform 2 (FHL1B) and isoform 3 (FHL1C) of human FHL1 (Uniprot Q13642).

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FHL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Four And A Half Lim Domains 1,Fcmsu,Fhl-1,Fhl1A,Fhl1B,Flh1A,Kyot,Rbmx1A,Rbmx1B,Slim,Slim-1,Slim1,Slimmer,Xmpma,Fhl1

Cellular Localization

Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Nucleus , Isoform 2: Nucleus , Cytoplasm , cytosol

Background

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Database

DATA IMAGES
GTX100266_WB_19011506_225.jpg

GTX100266 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX100266 diluted at 1:1000

GTX100266_39568_20160317_WB_M_Merged_19011506_409.jpg

GTX100266 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169