APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse muscle , mouse lung
Product Note
Based on sequence homology, GTX100266 is expected to recognize isoform 1 (FHL1A), isoform 4, and isoform 5, but not isoform 2 (FHL1B) and isoform 3 (FHL1C) of human FHL1 (Uniprot Q13642).
Predict Reactivity
Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FHL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240811
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
four and a half LIM domains 1 , FCMSU , FHL-1 , FHL1A , FHL1B , FLH1A , KYOT , RBMX1A , RBMX1B , SLIM , SLIM-1 , SLIM1 , SLIMMER , XMPMA
Cellular Localization
Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Nucleus , Isoform 2: Nucleus , Cytoplasm , cytosol
Background
LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX100266 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100266 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:1000.
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GTX100266 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
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REVIEW
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