GeneTex
United States (US)

FHL1 antibody

GTX124042_40814_IHC_M_2_18121410_422.jpg
GTX124042_40814_IHC_18121410_210.jpg
GTX124042_40814_WB_18121410_424.jpg
GTX124042_40814_IHC_M_18121410_332.jpg
GTX124042_40814_IHC_M_3_18121410_477.jpg

Cat No. GTX124042

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FHL1A antibody, FHL1B antibody, FLH1A antibody, KYOT antibody, MGC111107 antibody, SLIM1 antibody, SLIMMER antibody, XMPMA antibody, FHL1 antibody, "four-and-a-half Lin11, Isl-1 and Mec-3 domains 1 antibody", skeletal muscle LIM-protein1 antibody, four and a half LIM domains protein 1 antibody, LIM protein SLIMMER antibody, skeletal muscle LIM-protein 1 antibody, four and a half LIM domains 1 antibody

Cellular Localization

Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Nucleus , Isoform 2: Nucleus , Cytoplasm , cytosol ,

Background

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]
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Database

DATA IMAGES
GTX124042_40814_IHC_M_2_18121410_422.jpg

GTX124042 IHC-P Image

FHL1 antibody detects FHL1 protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
FHL1 antibody (GTX124042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124042_40814_IHC_18121410_210.jpg

GTX124042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using FHL1(GTX124042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124042_40814_WB_18121410_424.jpg

GTX124042 WB Image

Sample (30 ug of whole cell lysate)
A: Jurktat
12% SDS PAGE
GTX124042 diluted at 1:2000

GTX124042_40814_IHC_M_18121410_332.jpg

GTX124042 IHC-P Image

FHL1 antibody detects FHL1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
FHL1 antibody (GTX124042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124042_40814_IHC_M_3_18121410_477.jpg

GTX124042 IHC-P Image

FHL1 antibody detects FHL1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
FHL1 antibody (GTX124042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169