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FHL1 antibody

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042
Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042
Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042
Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042
Anti-FHL1 antibody used in Western Blot (WB). GTX124042

Cat No. GTX124042

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

four and a half LIM domains 1 , FCMSU , FHL-1 , FHL1A , FHL1B , FLH1A , KYOT , RBMX1A , RBMX1B , SLIM , SLIM-1 , SLIM1 , SLIMMER , XMPMA

Cellular Localization

Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Nucleus , Isoform 2: Nucleus , Cytoplasm , cytosol

Background

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042

GTX124042 IHC-P Image

FHL1 antibody detects FHL1 protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
FHL1 antibody (GTX124042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042

GTX124042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using FHL1(GTX124042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042

GTX124042 IHC-P Image

FHL1 antibody detects FHL1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
FHL1 antibody (GTX124042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124042

GTX124042 IHC-P Image

FHL1 antibody detects FHL1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
FHL1 antibody (GTX124042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL1 antibody used in Western Blot (WB). GTX124042

GTX124042 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX124042) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169