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FHL1 antibody

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199
Anti-FHL1 antibody used in Western Blot (WB). GTX33199
Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199
Anti-FHL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33199

Cat. No. GTX33199

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FHL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

four and a half LIM domains 1 , FCMSU , FHL-1 , FHL1A , FHL1B , FLH1A , KYOT , RBMX1A , RBMX1B , SLIM , SLIM-1 , SLIM1 , SLIMMER , XMPMA

Cellular Localization

Nucleus,Cytoplasm, cytosol

Background

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199

GTX33199 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33199 FHL1 antibody.
Dilution : 1:100

Anti-FHL1 antibody used in Western Blot (WB). GTX33199

GTX33199 WB Image

WB analysis of various sample lysates using GTX33199 FHL1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199

GTX33199 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX33199 FHL1 antibody.
Dilution : 1:100

Anti-FHL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33199

GTX33199 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33199 FHL1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399