APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:100 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
four and a half LIM domains 1 , FCMSU , FHL-1 , FHL1A , FHL1B , FLH1A , KYOT , RBMX1A , RBMX1B , SLIM , SLIM-1 , SLIM1 , SLIMMER , XMPMA
Cellular Localization
Nucleus,Cytoplasm, cytosol
Background
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
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GTX33199 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX33199 FHL1 antibody. Dilution : 1:100
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GTX33199 WB Image
WB analysis of various sample lysates using GTX33199 FHL1 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX33199 IHC-P Image
IHC-P analysis of human thyroid cancer tissue using GTX33199 FHL1 antibody. Dilution : 1:100
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GTX33199 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX33199 FHL1 antibody. Blue : DAPI
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REFERENCE
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REVIEW
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