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FHL1 antibody

Anti-FHL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33199
Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199
Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199
Anti-FHL1 antibody used in Western Blot (WB). GTX33199

Cat No. GTX33199

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

four and a half LIM domains 1 , FCMSU , FHL-1 , FHL1A , FHL1B , FLH1A , KYOT , RBMX1A , RBMX1B , SLIM , SLIM-1 , SLIM1 , SLIMMER , XMPMA

Cellular Localization

Nucleus,Cytoplasm, cytosol

Background

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]

Database

DATA IMAGES

Anti-FHL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33199

GTX33199 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33199 FHL1 antibody.
Blue : DAPI

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199

GTX33199 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33199 FHL1 antibody.
Dilution : 1:100

Anti-FHL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33199

GTX33199 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX33199 FHL1 antibody.
Dilution : 1:100

Anti-FHL1 antibody used in Western Blot (WB). GTX33199

GTX33199 WB Image

WB analysis of various samples using GTX33199 FHL1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329