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FHL2 antibody [AT21D11]

Anti-FHL2 antibody [AT21D11] used in Western Blot (WB). GTX57719
Anti-FHL2 antibody [AT21D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57719

Cat. No. GTX57719

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT21D11

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all FHL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT21D11 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FHL4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

four and a half LIM domains 2 , AAG11 , DRAL , FHL2 , SLIM3

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-FHL2 antibody [AT21D11] used in Western Blot (WB). GTX57719

GTX57719 WB Image

WB analysis of various samples using GTX57719 FHL2 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : SK-OV-3 whole cell lysate
Lane 5 : HT1080 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-FHL2 antibody [AT21D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57719

GTX57719 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57719 FHL2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399