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FHL2 antibody

Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114071
Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114071
Anti-FHL2 antibody used in IHC (Whole mount) (IHC-Wm). GTX114071
Anti-FHL2 antibody used in IHC (Whole mount) (IHC-Wm). GTX114071
Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114071
Anti-FHL2 antibody used in Western Blot (WB). GTX114071
Anti-FHL2 antibody used in Western Blot (WB). GTX114071

Cat. No. GTX114071

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all FHL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Human, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

four and a half LIM domains 2 , AAG11 , DRAL , FHL-2 , SLIM-3 , SLIM3

Cellular Localization

Cytoplasm , Nucleus

Background

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114071

GTX114071 IHC-P Image

FHL2 antibody detects FHL2 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
FHL2 antibody (GTX114071) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114071

GTX114071 IHC-P Image

FHL2 antibody detects FHL2 protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
FHL2 antibody (GTX114071) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL2 antibody used in IHC (Whole mount) (IHC-Wm). GTX114071

GTX114071 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using FHL2 antibody (GTX114071) at 1:200 dilution.

Anti-FHL2 antibody used in IHC (Whole mount) (IHC-Wm). GTX114071

GTX114071 IHC-Wm Image

FHL2 antibody detects FHL2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
FHL2 antibody (GTX114071) dilution: 1:100.
Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114071

GTX114071 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using FHL2 antibody (GTX114071) at 1:300 dilution.

Anti-FHL2 antibody used in Western Blot (WB). GTX114071

GTX114071 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish heart
12% SDS PAGE
GTX114071 diluted at 1:1000

Anti-FHL2 antibody used in Western Blot (WB). GTX114071

GTX114071 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL2 antibody (GTX114071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ahamad A et al. Front Neurosci 2020; 14 : 202 Early Dendritic Morphogenesis of Adult-Born Dentate Granule Cells Is Regulated by FHL2.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169