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FHL2 antibody

Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114072
Anti-FHL2 antibody used in Western Blot (WB). GTX114072
Anti-FHL2 antibody used in Western Blot (WB). GTX114072

Cat. No. GTX114072

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036926

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

four and a half LIM domains 2 , AAG11 , DRAL , FHL-2 , SLIM-3 , SLIM3

Cellular Localization

Cytoplasm , Nucleus

Background

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114072

GTX114072 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft , using FHL2(GTX114072) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FHL2 antibody used in Western Blot (WB). GTX114072

GTX114072 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL2 antibody (GTX114072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FHL2 antibody used in Western Blot (WB). GTX114072

GTX114072 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL2 antibody (GTX114072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169