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FHL5 antibody [N1C2]

Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031
Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031
Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031

Cat No. GTX107031

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950284

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

four and a half LIM domains 5 , 1700027G07Rik , ACT , FHL-5 , dJ393D12.2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031

GTX107031 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5(GTX107031) antibody at 1:500 dilution.

Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031

GTX107031 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5(GTX107031) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031

GTX107031 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Raji
12 % SDS PAGE
GTX107031 diluted at 1:1000

REFERENCE

There are currently no references for FHL5 antibody [N1C2] (GTX107031). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FHL5 antibody [N1C2] (GTX107031). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169