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FHL5 antibody [N1C2]

Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031
Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031
Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031

Cat. No. GTX107031

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FHL5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950284

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

four and a half LIM domains 5 , 1700027G07Rik , ACT , FHL-5 , dJ393D12.2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031

GTX107031 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5(GTX107031) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031

GTX107031 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5(GTX107031) antibody at 1:500 dilution.

Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031

GTX107031 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Raji
12 % SDS PAGE
GTX107031 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169