APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950284
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
four and a half LIM domains 5 , 1700027G07Rik , ACT , FHL-5 , dJ393D12.2
Cellular Localization
Nucleus
Background
The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031](/upload/website/prouct_img/middle/GTX107031/GTX107031_40016_IFA_19011506_414.jpg "Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031")
|
GTX107031 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5(GTX107031) antibody at 1:500 dilution.
|
![Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031](/upload/website/prouct_img/middle/GTX107031/GTX107031_IHC_19011506_835.jpg "Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031")
|
GTX107031 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5(GTX107031) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
![Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031](/upload/website/prouct_img/middle/GTX107031/GTX107031_WB_19011506_661.jpg "Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031")
|
GTX107031 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: Raji
12 % SDS PAGE
GTX107031 diluted at 1:1000
|
REFERENCE
There are currently no references for FHL5 antibody [N1C2] (GTX107031). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FHL5 antibody [N1C2] (GTX107031). Be the first to share your experience with this product.
![Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031](/upload/website/prouct_img/small/GTX107031/GTX107031_40016_IFA_19011506_414.jpg "Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031")
![Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031](/upload/website/prouct_img/small/GTX107031/GTX107031_IHC_19011506_835.jpg "Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031")
![Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031](/upload/website/prouct_img/small/GTX107031/GTX107031_WB_19011506_661.jpg "Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031")
![Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031](/upload/website/prouct_img/normal/GTX107031/GTX107031_40016_IFA_19011506_414.jpg "Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031")
![Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031](/upload/website/prouct_img/normal/GTX107031/GTX107031_IHC_19011506_835.jpg "Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031")
![Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031](/upload/website/prouct_img/normal/GTX107031/GTX107031_WB_19011506_661.jpg "Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031")
![Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507](/upload/website/prouct_img/small/GTX101507/GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg "Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507")
