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FHL5 antibody [N1C2]

Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031
Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031
Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031

Cat No. GTX107031

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
See all FHL5 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

four and a half LIM domains 5,1700027G07Rik,ACT,FHL-5,dJ393D12.2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-FHL5 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107031

GTX107031 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5(GTX107031) antibody at 1:500 dilution.
Anti-FHL5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX107031

GTX107031 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5(GTX107031) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-FHL5 antibody [N1C2] used in Western Blot (WB). GTX107031

GTX107031 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Raji
12 % SDS PAGE
GTX107031 diluted at 1:1000
REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169