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FIP200 antibody [N1N2], N-term

Anti-FIP200 antibody [N1N2], N-term used in Western Blot (WB). GTX107387
Anti-FIP200 antibody [N1N2], N-term used in Western Blot (WB). GTX107387
Anti-FIP200 antibody [N1N2], N-term used in Western Blot (WB). GTX107387
Anti-FIP200 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX107387

Cat. No. GTX107387

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FIP200 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

H1299 , mouse muscle

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FIP200. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951652

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB1 inducible coiled-coil 1 , ATG17 , CC1 , FIP200 , PPP1R131

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytosol,Lysosome

Background

The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-FIP200 antibody [N1N2], N-term used in Western Blot (WB). GTX107387

GTX107387 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FIP200 antibody [N1N2], N-term (GTX107387) diluted at 1:500.

Anti-FIP200 antibody [N1N2], N-term used in Western Blot (WB). GTX107387

GTX107387 WB Image

FIP200 antibody detects RIP200 protein by Western blot analysis.
A. 30 μg H1299 whole cell lysate/extract
5 % SDS-PAGE
RB1CC1 antibody (GTX107387) dilution: 1:1000

Anti-FIP200 antibody [N1N2], N-term used in Western Blot (WB). GTX107387

GTX107387 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX107387 diluted at 1:500

Anti-FIP200 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX107387

GTX107387 IHC-P Image

FIP200 antibody [N1N2], N-term detects FIP200 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FIP200 antibody [N1N2], N-term (GTX107387) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chen D et al. Biochem Biophys Res Commun 2013; 432 (2) : 226-30 CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable.
Application : WB, ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169