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FIP200 antibody

Overexpression
Anti-FIP200 antibody used in Western Blot (WB). GTX129094
Anti-FIP200 antibody used in Western Blot (WB). GTX129094

Cat. No. GTX129094

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FIP200 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human RB1CC1-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FIP200. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885891

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB1 inducible coiled-coil 1 , ATG17 , CC1 , FIP200 , PPP1R131

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytosol,Lysosome

Background

The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-FIP200 antibody used in Western Blot (WB). GTX129094

GTX129094 WB Image

Overexpression

FIP200 antibody detects FIP200 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human FIP200-transfected 293T cells
5 % SDS-PAGE
FIP200 antibody (GTX129094) dilution: 1:500

Anti-FIP200 antibody used in Western Blot (WB). GTX129094

GTX129094 WB Image

FIP200 antibody detects FIP200 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
FIP200 antibody (GTX129094) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169