FIS1 antibody [GT1188]

Cat. No. GTX00950

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1188
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all FIS1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
IP	1:50 - 1:200

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human TTC11.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fission, mitochondrial 1 , CGI135 , FIS1 , TTC11

Cellular Localization

Mitochondrion outer membrane,Peroxisome membrane

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]

Database

Gene ID: 51024 FIS1
UniProt: Q9Y3D6 FIS1

Research Area

DATA IMAGES

GTX00950 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT1188] (GTX00950) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX00950 IP Image

IP analysis of HeLa cell lysate using GTX00950 FIS1 antibody [GT1188].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

GTX00950 WB Image

WB analysis of various samples using GTX00950 FIS1 antibody [GT1188].
Dilution : 1:1000
Loading : 25μg

GTX00950 WB Image

WB analysis of wild-type (WT) and FIS1 knockout (KO) HeLa cell extracts using GTX00950 FIS1 antibody [GT1188].
Dilution : 1:1000
Laoding : 25μg

GTX00950 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00950 FIS1 antibody [GT1188].
Dilution : 1:100

GTX00950 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX00950 FIS1 antibody [GT1188].
Dilution : 1:100

GTX00950 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX00950 FIS1 antibody [GT1188].
Dilution : 1:100

GTX00950 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00950 FIS1 antibody [GT1188].
Red : Primary antibody
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

FIS1 antibody [GT1188]

Cat. No. GTX00950

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX00950 WB Image

GTX00950 IP Image

GTX00950 WB Image

GTX00950 WB Image

GTX00950 IHC-P Image

GTX00950 IHC-P Image

GTX00950 IHC-P Image

GTX00950 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.