APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NCI-H929 , HeLa
Predict Reactivity
Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
3.55 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human FIS1 Recombinant protein.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888237
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
fission, mitochondrial 1 , CGI-135 , TTC11
Cellular Localization
Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein
Background
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992](/templates/default/js/lightbox/images/loading.gif "Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992")
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GTX630992 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT12112] (GTX630992) diluted at 1:500.
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GTX630992 WB Image
FIS1 antibody [GT12112] detects FIS1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15 % SDS-PAGE, and blotted with FIS1 antibody [GT12112] (GTX630992) diluted by 1:1000
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GTX630992 ICC/IF Image
FIS1 antibody [GT12112] detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody [GT12112] (GTX630992) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for FIS1 antibody [GT12112] (GTX630992). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FIS1 antibody [GT12112] (GTX630992). Be the first to share your experience with this product.
![Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992](/upload/website/prouct_img/small/GTX630992/GTX630992_41750_20161013_WB_shRNA_watermark_18121411_317.jpg "Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992")
![Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992](/upload/website/prouct_img/small/GTX630992/GTX630992_41750_WB_18121411_546.jpg "Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992")
![Anti-FIS1 antibody [GT12112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630992](/upload/website/prouct_img/small/GTX630992/GTX630992_41750_IFA_18121411_614.jpg "Anti-FIS1 antibody [GT12112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630992")
![Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992](/upload/website/prouct_img/normal/GTX630992/GTX630992_41750_20161013_WB_shRNA_watermark_18121411_317.jpg "Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992")
![Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992](/upload/website/prouct_img/normal/GTX630992/GTX630992_41750_WB_18121411_546.jpg "Anti-FIS1 antibody [GT12112] used in Western Blot (WB). GTX630992")
![Anti-FIS1 antibody [GT12112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630992](/upload/website/prouct_img/normal/GTX630992/GTX630992_41750_IFA_18121411_614.jpg "Anti-FIS1 antibody [GT12112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630992")