APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human FIS1 Recombinant protein.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888235
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fission, mitochondrial 1 , CGI-135 , TTC11
Cellular Localization
Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein
Background
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/templates/default/js/lightbox/images/loading.gif "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
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GTX630983 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500.
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GTX630983 ICC/IF Image
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX630983) diluted at 1:1000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX630983 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630983 WB Image
FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX630983) diluted by 1:1000.
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GTX630983 ICC/IF Image
FIS1 antibody [GT4211] detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.
Green: FIS1 protein stained by FIS1 antibody [GT4211] (GTX630983) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for FIS1 antibody [GT4211] (GTX630983). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FIS1 antibody [GT4211] (GTX630983). Be the first to share your experience with this product.
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/upload/website/prouct_img/small/GTX630983/GTX630983_41904_20161013_WB_shRNA_watermark_w_23061202_286.webp "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983](/upload/website/prouct_img/small/GTX630983/GTX630983_41904_20141205_IFA_w_23061202_450.webp "Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/upload/website/prouct_img/small/GTX630983/GTX630983_41904_20191004_WB_R_w_23061202_888.webp "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/upload/website/prouct_img/small/GTX630983/GTX630983_41904_20141121_WB_w_23061202_415.webp "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983](/upload/website/prouct_img/small/GTX630983/GTX630983_41904_20150821_IFA_w_23061202_538.webp "Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20161013_WB_shRNA_watermark_w_23061202_286.webp "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983](/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20141205_IFA_w_23061202_450.webp "Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20191004_WB_R_w_23061202_888.webp "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983](/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20141121_WB_w_23061202_415.webp "Anti-FIS1 antibody [GT4211] used in Western Blot (WB). GTX630983")
![Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983](/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20150821_IFA_w_23061202_538.webp "Anti-FIS1 antibody [GT4211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630983")