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FIS1 antibody [GT9810]

KO/KD
Anti-FIS1 antibody [GT9810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631209
Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209
Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209
Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

Cat No. GTX631209

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT9810

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all FIS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:20000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

HeLa , NCI-H929

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human FIS1 Recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fission, mitochondrial 1 , CGI-135 , TTC11

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FIS1 antibody [GT9810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631209

GTX631209 ICC/IF Image

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX631209) diluted at 1:20000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

GTX631209 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT9810] (GTX631209) diluted at 1:500.

Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

GTX631209 WB Image

FIS1 antibody [GT9810] detects FIS1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15 % SDS-PAGE, and blotted with FIS1 antibody [GT9810] (GTX631209) diluted by 1:1000

Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

GTX631209 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT9810] (GTX631209) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang J et al. Exp Mol Med 2018; 50 (11) : 146 Small molecule natural compound agonist of SIRT3 as a therapeutic target for the treatment of intervertebral disc degeneration.
Application : WB
Reactivity : Human
Wang J et al. Int J Biol Sci 2018; 14 (13) : 1873-1882 SIRT3 Activation by Dihydromyricetin Suppresses Chondrocytes Degeneration via Maintaining Mitochondrial Homeostasis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169