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FIS1 antibody [GT9810]

KO/KD
Anti-FIS1 antibody [GT9810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631209
Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209
Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209
Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

Cat. No. GTX631209

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT9810

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FIS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:20000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

NCI-H929 , HeLa

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human FIS1 Recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888243

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fission, mitochondrial 1 , CGI-135 , TTC11

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FIS1 antibody [GT9810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631209

GTX631209 ICC/IF Image

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX631209) diluted at 1:20000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

GTX631209 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT9810] (GTX631209) diluted at 1:500.

Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

GTX631209 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT9810] (GTX631209) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-FIS1 antibody [GT9810] used in Western Blot (WB). GTX631209

GTX631209 WB Image

FIS1 antibody [GT9810] detects FIS1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15 % SDS-PAGE, and blotted with FIS1 antibody [GT9810] (GTX631209) diluted by 1:1000

REFERENCE

Application Reference

Sotty J et al. Environ Int 2020; 142 : 105830 Mitochondrial alterations triggered by repeated exposure to fine (PM2.5-0.18) and quasi-ultrafine (PM0.18) fractions of ambient particulate matter.
Application : WB
Reactivity : Human
Wang J et al. Exp Mol Med 2018; 50 (11) : 146 Small molecule natural compound agonist of SIRT3 as a therapeutic target for the treatment of intervertebral disc degeneration.
Application : WB
Reactivity : Human
Wang J et al. Int J Biol Sci 2018; 14 (13) : 1873-1882 SIRT3 Activation by Dihydromyricetin Suppresses Chondrocytes Degeneration via Maintaining Mitochondrial Homeostasis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations