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FIS1 antibody

Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010

Cat No. GTX111010

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji , Mouse brain , mouse liver , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human FIS1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950286

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fission, mitochondrial 1 , CGI-135 , TTC11

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
15% SDS PAGE
GTX111010 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX111010 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010

GTX111010 ICC/IF Image

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TTC11 antibody (GTX111010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

FIS1 antibody Cat No. GTX111010

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 6 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Redox Biol in 2019.PMID: 30388684
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28220896
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Physiol Rep in 2018.PMID: 29932506
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2019.PMID: 30761259
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015.PMID: 26717518
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32560241
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf