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FIS1 antibody

Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010

Cat No. GTX111010

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji , Mouse brain , mouse liver , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human FIS1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fission, mitochondrial 1 , CGI-135 , TTC11

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
15% SDS PAGE
GTX111010 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX111010 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010

GTX111010 ICC/IF Image

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TTC11 antibody (GTX111010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169