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FIS1 antibody

KO/KD
Anti-FIS1 antibody used in Immunoprecipitation (IP). GTX55624
Anti-FIS1 antibody used in Western Blot (WB). GTX55624
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55624
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55624
Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55624
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55624
Anti-FIS1 antibody used in Western Blot (WB). GTX55624

Cat. No. GTX55624

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all FIS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human FIS1 (NP_057152.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fission, mitochondrial 1 , CGI-135 , TTC11

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-FIS1 antibody used in Immunoprecipitation (IP). GTX55624

GTX55624 IP Image

IP analysis of MCF-7 cell lysate using GTX55624 FIS1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-FIS1 antibody used in Western Blot (WB). GTX55624

GTX55624 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55624 FIS1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55624

GTX55624 IHC-P Image

IHC-P analysis of rat heart tissue using GTX55624 FIS1 antibody.
Dilution : 1:100

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55624

GTX55624 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX55624 FIS1 antibody.
Dilution : 1:100

Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55624

GTX55624 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55624 FIS1 antibody.
Blue : DAPI
Dilution : 1:50

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55624

GTX55624 IHC-P Image

IHC-P analysis of rat heart tissue using GTX55624 FIS1 antibody.
Dilution : 1:100

Anti-FIS1 antibody used in Western Blot (WB). GTX55624

GTX55624 WB Image

WB analysis of 293T cell lysate using GTX55624 FIS1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399