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FKBP12 antibody [3B3]

Overexpression
Anti-FKBP12 antibody [3B3] used in Western Blot (WB). GTX84492
Anti-FKBP12 antibody [3B3] used in Flow cytometry (FACS). GTX84492
Anti-FKBP12 antibody [3B3] used in IHC (Paraffin sections) (IHC-P). GTX84492
Anti-FKBP12 antibody [3B3] used in Western Blot (WB). GTX84492
Anti-FKBP12 antibody [3B3] used in IHC (Paraffin sections) (IHC-P). GTX84492

Cat. No. GTX84492

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3B3

Isotype

 IgG2b

Application

 WB, IHC-P, FACS

Reactivity

 Human, Dog
Package
100 μl ($519)
See all FKBP12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human FKBP1A (NP_000792) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FKBP prolyl isomerase 1A , FKBP-12 , FKBP-1A , FKBP1 , FKBP12 , PKC12 , PKCI2 , PPIASE

Cellular Localization

Cytoplasm, cytosol

Background

May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Database

Research Area

DATA IMAGES

Anti-FKBP12 antibody [3B3] used in Western Blot (WB). GTX84492

GTX84492 WB Image

WB analysis of various cell lines using GTX84492 FKBP12 antibody [3B3].
Loading : 10 ug per lane
Dilution : 1:200

Anti-FKBP12 antibody [3B3] used in Flow cytometry (FACS). GTX84492

GTX84492 FACS Image

FACS analysis of HEK293T cells transfected with either FKBP12 plasmid(Red) or empty vector control plasmid(Blue) using GTX84492 FKBP12 antibody [3B3].

Anti-FKBP12 antibody [3B3] used in IHC (Paraffin sections) (IHC-P). GTX84492

GTX84492 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84492 FKBP12 antibody [3B3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FKBP12 antibody [3B3] used in Western Blot (WB). GTX84492

GTX84492 WB Image

Overexpression

WB analysis of HEK293T cells transfected with FKBP12 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84492 FKBP12 antibody [3B3].
Loading : 5 ug per lane

Anti-FKBP12 antibody [3B3] used in IHC (Paraffin sections) (IHC-P). GTX84492

GTX84492 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84492 FKBP12 antibody [3B3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519