GeneTex
United States (US)

FKBP12 antibody

GTX31576_2761_WB_20180221_18121410_780.jpg
GTX31576_218_ICC-IF_20180221_18121410_802.jpg
GTX31576_217_ICC-IF_20180221_18121410_777.jpg

Cat No. GTX31576

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

A-431 Cell Slide(GTX25525) , A431 Cell Lysate(GTX27909)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12.The immunogen is located within the first 50 amino acids of FKBP12.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fk506 Binding Protein 1A,Fkbp-12,Fkbp-1A,Fkbp1,Fkbp12,Pkc12,Pkci2,Ppiase,Fkbp1A

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008]

Database

DATA IMAGES
GTX31576_2761_WB_20180221_18121410_780.jpg

GTX31576 WB Image

WB analysis of A431 cell lysate using GTX31576 FKBP12 antibody.
Working concentration : 1 μg/ml

GTX31576_218_ICC-IF_20180221_18121410_802.jpg

GTX31576 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31576 FKBP12 antibody.
Working concentration : 20 μg/ml

GTX31576_217_ICC-IF_20180221_18121410_777.jpg

GTX31576 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31576 FKBP12 antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329