GeneTex
United States (US)

FKBP25 antibody [N1C3]

GTX105608_WB_19011506_276.jpg
GTX105608_40142_WB_M_brain_19011506_451.jpg

Cat No. GTX105608

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FKBP25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FKBP-25 antibody, PPIase antibody, FKBP3 antibody, FK506-binding protein 3 (25kD) antibody, 25 kDa FKBP antibody, "FK506-binding protein 25, T-cell antibody", peptidyl-prolyl cis-trans isomerase FKBP3 antibody, 25 kDa FK506-binding protein antibody, rapamycin-selective 25 kDa immunophilin antibody, rotamase antibody, PPIase FKBP3 antibody, immunophilin FKBP25 antibody, rapamycin binding protein antibody, "FK506 binding protein 3, 25kDa antibody"

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. [provided by RefSeq]

Database

DATA IMAGES
GTX105608_WB_19011506_276.jpg

GTX105608 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
12% SDS PAGE
GTX105608 diluted at 1:1000

GTX105608_40142_WB_M_brain_19011506_451.jpg

GTX105608 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105608 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169