GeneTex
United States (US)

FKBP4 antibody, C-term

GTX88487_2270_WB_18122102_874.jpg
GTX88487_932_IHC-P_18122102_122.jpg
GTX88487_931_IHC-P_18122102_113.jpg

Cat No. GTX88487

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88487-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-DHPTDTEMKEEQKSN, from the C Terminus of the protein sequence according to NP_002005.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HBI Antibody , HSP56 Antibody , FKBP59 Antibody , P52 Antibody , FKBP52 Antibody , PPIASE Antibody , FKBP51 Antibody

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]
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Database

Research Area

DATA IMAGES
GTX88487_2270_WB_18122102_874.jpg

GTX88487 WB Image

WB analysis of human placenta lysate using GTX88487 FKBP4 antibody, C-term.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

GTX88487_932_IHC-P_18122102_122.jpg

GTX88487 IHC-P Image

IHC-P analysis of human kidney using GTX88487 FKBP4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88487_931_IHC-P_18122102_113.jpg

GTX88487 IHC-P Image

IHC-P analysis of human cerebellum using GTX88487 FKBP4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml