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FKBP5 antibody [3E9]

KO/KD Overexpression
Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491
Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491
Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491
Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491
Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491
Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491
Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491
Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491
Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491
Anti-FKBP5 antibody [3E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84491

Cat. No. GTX84491

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all FKBP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human FKBP5 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FKBP prolyl isomerase 5 , AIG6 , FKBP51 , FKBP54 , P54 , PPIase , Ptg-10

Cellular Localization

Cytoplasm,Nucleus

Background

Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.

Database

Research Area

DATA IMAGES

Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491

GTX84491 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84491 FKBP5 antibody [3E9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491

GTX84491 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84491 FKBP5 antibody [3E9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491

GTX84491 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84491 FKBP5 antibody [3E9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491

GTX84491 IHC-P Image

IHC-P analysis of colon tissue using GTX84491 FKBP5 antibody [3E9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491

GTX84491 IHC-P Image

IHC-P analysis of prostate tissue using GTX84491 FKBP5 antibody [3E9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491

GTX84491 WB Image

WB analysis of various cell lines using GTX84491 FKBP5 antibody [3E9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491

GTX84491 WB Image

Overexpression

WB analysis of HEK293T cells transfected with FKBP5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84491 FKBP5 antibody [3E9].
Loading : 5 ug per lane

Anti-FKBP5 antibody [3E9] used in IHC (Paraffin sections) (IHC-P). GTX84491

GTX84491 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84491 FKBP5 antibody [3E9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-FKBP5 antibody [3E9] used in Western Blot (WB). GTX84491

GTX84491 WB Image

KO/KD

WB analysis of wild type and FKBP5 knock out HeLa cell lysate(10μg per lane) using GTX84491 FKBP5 antibody [3E9].
Dilution : 1:500

Anti-FKBP5 antibody [3E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84491

GTX84491 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with FKBP5 plasmid using GTX84491 FKBP5 antibody [3E9].

REFERENCE

Application Reference

Lee EH et al. Nat Commun 2021; 12 (1) : 6682 Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in mice.
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519