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FLASH antibody

Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420
Anti-FLASH antibody used in Western Blot (WB). GTX28420
Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420

Cat. No. GTX28420

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($479)
See all FLASH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5μg/ml
ICC/IF 10μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

223 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 1253-1268 of human FLASH (accession no. AF165161). This sequence differs by one amino acid from that of mouse.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP8AP2 , CED4 , FLASH , RIP25 , caspase 8 associated protein 2

Cellular Localization

Cytoplasm,Nucleus,Nucleus, PML body,Mitochondrion

Background

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420

GTX28420 ICC/IF Image

Immunocytochemical staining of HeLa cells with Rabbit anti Human FLASH (GTX28420)

Anti-FLASH antibody used in Western Blot (WB). GTX28420

GTX28420 WB Image

Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/ml.

Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420

GTX28420 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX28420 FLASH antibody.
Dilution : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479