GeneTex
United States (US)

FLASH antibody

FLASH-antibody-GTX28420-WB-1_18121410_778.jpg
FLASH-antibody__GTX28420-1_18121410_985.jpg

Cat No. GTX28420

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

For WB: Use at a dilution of 1:1,000-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

223 kDa. ( Note )

Positive Control

HeLa cell lysate
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

1221~1270a.a of human FLASH(AAD45157). This sequence differs by one amino acid from that of mouse.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CASP8AP2, KIAA1315, 606880, CED-4, CED4, FLASH, Q9UKL3, RIP25, FLJ11208, 9994, CED 4

Background

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).

Database

Research Area

DATA IMAGES
FLASH-antibody-GTX28420-WB-1_18121410_778.jpg

GTX28420 WB Image

Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/ml.

FLASH-antibody__GTX28420-1_18121410_985.jpg

GTX28420 ICC/IF Image

Immunocytochemical staining of HeLa cells with Rabbit anti Human FLASH (GTX28420)

Package List Price ($)
$ 349