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FLI1 antibody [HL1805]

Recombinant antibody Orthogonal
Anti-FLI1 antibody [HL1805] used in Western Blot (WB). GTX637457
Anti-FLI1 antibody [HL1805] used in Immunoprecipitation (IP). GTX637457
Anti-FLI1 antibody [HL1805] used in Western Blot (WB). GTX637457

Cat. No. GTX637457

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1805

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FLI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
IP Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

This antibody is specific for human FLI1 protein, and it does not cross react with human ERG protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human FLI1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-FLI1 antibody [HL1805] used in Western Blot (WB). GTX637457

GTX637457 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody [HL1805] (GTX637457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FLI1 antibody [HL1805] used in Immunoprecipitation (IP). GTX637457

GTX637457 IP Image

Immunoprecipitation of FLI1 protein from Raji whole cell extract using 5 μg of FLI1 antibody [HL1805] (GTX637457).
Western blot analysis was performed using FLI1 antibody [HL1805] (GTX637457).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-FLI1 antibody [HL1805] used in Western Blot (WB). GTX637457

GTX637457 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody [HL1805] (GTX637457) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations