APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
Note :
IHC-P
Antigen retrieval : heat mediated tissue sections in 10mM Tris with 1mM EDTA (pH 9.0) followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Positive Control
Human tonsil, Ewing's sarcoma (EWS), or angiosarcoma
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% Sodium Azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (200-400 aa) of human FLI1 protein
Purification
Purified by Protein A/G
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EWSR2,FLI1,Fli1 protooncogene, ETS transcription factor,SIC1,Fli-1 proto-oncogene, ETS transcription factor
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Database
Research Area
DATA IMAGES
|
GTX05013 IHC-P Image
IHC-P analysis of human Ewing Sarcoma tissues using GTX05013 FLI1 antibody [rFLI1/9614]. Antigen retrieval : Heat mediated antigen retrieval in Tris/EDTA buffer (pH 9.0).
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REFERENCE
There are currently no references for FLI1 antibody [rFLI1/9614] (GTX05013). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FLI1 antibody [rFLI1/9614] (GTX05013). Be the first to share your experience with this product.