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FLI1 antibody

Anti-FLI1 antibody used in Western Blot (WB). GTX112937
Anti-FLI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112937
Anti-FLI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112937
Anti-FLI1 antibody used in Immunoprecipitation (IP). GTX112937
Anti-FLI1 antibody used in Western Blot (WB). GTX112937

Cat. No. GTX112937

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all FLI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

rat kidney , BCL-1

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1

Cellular Localization

Nucleus

Background

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Database

Research Area

DATA IMAGES

Anti-FLI1 antibody used in Western Blot (WB). GTX112937

GTX112937 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (GTX112937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FLI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112937

GTX112937 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1(GTX112937) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FLI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112937

GTX112937 ICC/IF Image

FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FLI1 protein stained by FLI1 antibody (GTX112937) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-FLI1 antibody used in Immunoprecipitation (IP). GTX112937

GTX112937 IP Image

FLI1 antibody immunoprecipitates FLI1 protein in IP experiments.
IP samples: Raji whole cell extract
A. 40 μg Raji whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FLI1 protein by 4 μg FLI1 antibody (GTX112937)
10 % SDS-PAGE
The immunoprecipitated FLI1 protein was detected by FLI1 antibody (GTX112937) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-FLI1 antibody used in Western Blot (WB). GTX112937

GTX112937 WB Image

FLI1 antibody detects FLI1 protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
10% SDS-PAGE
FLI1 antibody (GTX112937) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169