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FLI1 antibody

Anti-FLI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31888
Anti-FLI1 antibody used in Western Blot (WB). GTX31888

Cat No. GTX31888

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , Jurkat Cell Lysate(GTX27899)

Specificity/Sensitivity

At least four isoforms of FLI1 are known to exist; this antibody will detect all four.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1.The immunogen is located within the last 50 amino acids of FLI1 .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-FLI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31888

GTX31888 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31888 FLI1 antibody.
Working concentration : 5 μg/ml

Anti-FLI1 antibody used in Western Blot (WB). GTX31888

GTX31888 WB Image

WB analysis of Jurkat cell lysate using GTX31888 FLI1 antibody.
Dilution : 0.5μg/ml (Lane A) and 1 μg/ml (Lane B)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339