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FLI1 antibody

Anti-FLI1 antibody used in Immunoprecipitation (IP). GTX32607
Anti-FLI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32607
Anti-FLI1 antibody used in Western Blot (WB). GTX32607
Anti-FLI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32607

Cat. No. GTX32607

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399)
See all FLI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-FLI1 antibody used in Immunoprecipitation (IP). GTX32607

GTX32607 IP Image

IP analysis of Jurkat cell lysate using GTX32607 FLI1 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-FLI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32607

GTX32607 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32607 FLI1 antibody.
Dilution : 1:100

Anti-FLI1 antibody used in Western Blot (WB). GTX32607

GTX32607 WB Image

WB analysis of Jurkat cell lysate using GTX32607 FLI1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-FLI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32607

GTX32607 IHC-P Image

IHC-P analysis of human uterine cancer tissue using GTX32607 FLI1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399