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FLIP antibody [5D8]

Anti-FLIP antibody [5D8] used in Western Blot (WB). GTX53717

Cat No. GTX53717

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D8

Isotype

 IgG3

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($339)
See all FLIP products

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human FLIP (1-376aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CASP8 and FADD like apoptosis regulator , CASH , CASP8AP1 , CLARP , Casper , FLAME , FLAME-1 , FLAME1 , FLIP , I-FLICE , MRIT , c-FLIP , c-FLIPL , c-FLIPR , c-FLIPS , cFLIP

Background

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-FLIP antibody [5D8] used in Western Blot (WB). GTX53717

GTX53717 WB Image

WB analysis of lysates from mouse kidney, MCF7 and rat spleen (40ug per lane) using FLIP antibody at a dilution of 1:500.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339