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FLIP antibody [7A3D12]

Anti-FLIP antibody [7A3D12] used in Western Blot (WB). GTX60781
Anti-FLIP antibody [7A3D12] used in ELISA (ELISA). GTX60781
Anti-FLIP antibody [7A3D12] used in Flow cytometry (FACS). GTX60781
Anti-FLIP antibody [7A3D12] used in IHC (Paraffin sections) (IHC-P). GTX60781
Anti-FLIP antibody [7A3D12] used in IHC (Paraffin sections) (IHC-P). GTX60781

Cat. No. GTX60781

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7A3D12

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all FLIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human FLIP (AA: 100-251) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP8 and FADD like apoptosis regulator , CASH , CASP8AP1 , CLARP , Casper , FLAME , FLAME-1 , FLAME1 , FLIP , I-FLICE , MRIT , c-FLIP , c-FLIPL , c-FLIPR , c-FLIPS , cFLIP

Background

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-FLIP antibody [7A3D12] used in Western Blot (WB). GTX60781

GTX60781 WB Image

WB analysis of Jurkat (1), 3T3L1 (2) and Raji (3) cell lysate using GTX60781 FLIP antibody [7A3D12].

Anti-FLIP antibody [7A3D12] used in ELISA (ELISA). GTX60781

GTX60781 ELISA Image

ELISA analysis of antigen using GTX60781 FLIP antibody [7A3D12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-FLIP antibody [7A3D12] used in Flow cytometry (FACS). GTX60781

GTX60781 FACS Image

FACS analysis of JURKAT cells using GTX60781 FLIP antibody [7A3D12].
Green : FLIP
Red : negative control

Anti-FLIP antibody [7A3D12] used in IHC (Paraffin sections) (IHC-P). GTX60781

GTX60781 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60781 FLIP antibody [7A3D12].

Anti-FLIP antibody [7A3D12] used in IHC (Paraffin sections) (IHC-P). GTX60781

GTX60781 IHC-P Image

IHC-P analysis of esophagus cancer tissue using GTX60781 FLIP antibody [7A3D12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399