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FLIP antibody [N1C1]

Comparison
Anti-FLIP antibody [N1C1] used in Western Blot (WB). GTX113047
Anti-FLIP antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113047

Cat. No. GTX113047

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FLIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FLIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727706

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP8 and FADD like apoptosis regulator , CASH , CASP8AP1 , CLARP , Casper , FLAME , FLAME-1 , FLAME1 , FLIP , I-FLICE , MRIT , c-FLIP , c-FLIPL , c-FLIPR , c-FLIPS , cFLIP

Background

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

Database

Research Area

DATA IMAGES

Anti-FLIP antibody [N1C1] used in Western Blot (WB). GTX113047

GTX113047 WB Image

Comparison

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with FLIP antibody [N1C1] (GTX113047) diluted at 1:500 and competitor's antibody (sc-8347) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-FLIP antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113047

GTX113047 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FLIP(GTX113047) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yuan Q et al. Cancers (Basel) 2022; 14 (14) Pulmonary Delivery of Extracellular Vesicle-Encapsulated Dinaciclib as an Effective Lung Cancer Therapy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169