APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 5μg/ml |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes all human, mouse, and rat FLIP splice variants.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide corresponding to aa 2-18 of human FLIP (accession no. AAC51622). This sequence is identical in all FLIP splice variants.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
RRID
AB_368661
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CASH , CASP8 and FADD like apoptosis regulator , CASP8AP1 , CFLAR , CLARP , Casper , FLAME , FLAME1 , FLIP , IFLICE , MRIT , cFLIP , cFLIPL , cFLIPR , cFLIPS
Background
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX28421 WB Image
Western blot analysis of FLIP in K562 cell lysate with FLIP antibody (GTX28421) at (A) 1 and (B) 2μg/ml.
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GTX28421 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX28421 FLIP antibody.
Dilution : 20 μg/ml
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GTX28421 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX28421 FLIP antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
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GTX28421 ICC/IF Image
Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody (GTX28421) at 5μg/ml.
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GTX28421 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX28421 FLIP antibody.
Dilution : 5 μg/ml
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GTX28421 WB Image
WB analysis of (A) HeLa cell and (B) mouse liver tissue lysate using GTX28421 FLIP antibody.
Dilution : 1 μg/ml
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GTX28421 WB Image
The data was published in the 2016 in Oncotarget. PMID: 27564258
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REFERENCE
REVIEW
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