GeneTex
United States (US)

FLIP antibody

FLIP-antibody_WB_GTX28421-2_18121410_705.jpg
FLIP-antibody_WB_GTX28421-1_18121410_468.jpg

Cat No. GTX28421

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Western blot at1:500 to 1:1000 dilution.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to amino acids 2 to 18 of human FLIP2. The sequence is identical in all FLIP splice variants.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Q01543, SIC1, SIC-1, FLI1, FLIP, EWSR2, 2313, 193067, CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, SIC 1, c FLIP, FLAME 1, I FLICE, c FLIPL, c FLIPR, c FLIPS, cFLIP, IFLICE, cFLIPL, cFLIPR, cFLIPS

Cellular Localization

Cytoplasmic

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively (1,2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and-10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively (8).

Database

Research Area

DATA IMAGES
FLIP-antibody_WB_GTX28421-2_18121410_705.jpg

GTX28421 WB Image

Western blot analysis of FLIP in K562 cell lysate with FLIP antibody (GTX28421) at (A) 1 and (B) 2μg/ml.

FLIP-antibody_WB_GTX28421-1_18121410_468.jpg

GTX28421 ICC/IF Image

Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody (GTX28421) at 5μg/ml.

REFERENCE