For WB: Use at a dilution of 500 - 1:1,000. Optimal concentrations/dilutions should be determined by the researcher.
Whole cell lysate NIH3T3 cells
FLIP has two forms, a short form, FLIPS, and long form, FLIPL; this antibody recognizes the FLIPL form.
PBS, pH 7.4 with 0.02% sodium azide as preservative
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5mg/ml(Please refer to the vial label for the specific concentration.)
Peptide corresponding to C-terminus of mouse FLIPL/CASH alpha form
Affinity purified with antigen
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Q01543, SIC1, SIC-1, FLI1, FLIP, EWSR2, 2313, 193067, SIC 1
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively (1,2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT (3-8). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and-10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
GTX28422 WB Image
Western blot analysis of mFLIP in NIH/3T3 whole cell lysate with anti-mFLIP (CT) (GTX28422) at 2ug/ml.
GTX28422 ICC/IF Image
Immunocytochemical staining of NIH/3T3 cells with mFLIP antibody (GTX28422) at 5ug/ml