GeneTex
United States (US)

FLIP antibody

FLIP-antibody_WB_GTX28422-1_18121410_694.jpg
FLIP-antibody_ICCIF_GTX28422-1_18121410_159.jpg

Cat No. GTX28422

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

For WB: Use at a dilution of 500 - 1:1,000. Optimal concentrations/dilutions should be determined by the researcher.

Calculated MW

55 kDa. ( Note )

Positive Control

Whole cell lysate NIH3T3 cells

Specificity/Sensitivity

FLIP has two forms, a short form, FLIPS, and long form, FLIPL; this antibody recognizes the FLIPL form.
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4 with 0.02% sodium azide as preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide corresponding to C-terminus of mouse FLIPL/CASH alpha form

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Q01543, SIC1, SIC-1, FLI1, FLIP, EWSR2, 2313, 193067, SIC 1

Cellular Localization

Cytoplasmic

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively (1,2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT (3-8). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and-10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

Database

Research Area

DATA IMAGES
FLIP-antibody_WB_GTX28422-1_18121410_694.jpg

GTX28422 WB Image

Western blot analysis of mFLIP in NIH/3T3 whole cell lysate with anti-mFLIP (CT) (GTX28422) at 2ug/ml.

FLIP-antibody_ICCIF_GTX28422-1_18121410_159.jpg

GTX28422 ICC/IF Image

Immunocytochemical staining of NIH/3T3 cells with mFLIP antibody (GTX28422) at 5ug/ml