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FLRT1 antibody [N1N3]

Anti-FLRT1 antibody [N1N3] used in Western Blot (WB). GTX105597
Anti-FLRT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105597
Anti-FLRT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105597

Cat. No. GTX105597

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FLRT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

H1299 , HeLaS3

Predict Reactivity

Mouse, Rat, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human FLRT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950290

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibronectin leucine rich transmembrane protein 1 , SPG68

Background

This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. The family members may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. The encoded protein shares sequence similarity with two other family members, FLRT2 and FLRT3. This gene is expressed in kidney and brain. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FLRT1 antibody [N1N3] used in Western Blot (WB). GTX105597

GTX105597 WB Image

Sample (30μg whole cell lysate)
A: H1299
B: HeLa S3(GTX14654)
7.5% SDS PAGE
GTX105597 diluted at 1:1000

Anti-FLRT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105597

GTX105597 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using FLRT1(GTX105597) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FLRT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105597

GTX105597 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using FLRT1(GTX105597) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169