GeneTex
United States (US)

FLT3 antibody

GTX31863_3010_WB_20180221_18121410_734.jpg
GTX31863_1503_IHC-P_20180221_18121410_517.jpg

Cat No. GTX31863

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

Human Kidney Tissue Slide , 3T3 Cell Lysate

Specificity/Sensitivity

At least three isoforms of FLT3 are known to exist; this antibody will detect all isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FLT3 antibody was raised against an 18 amino acid synthetic peptide near the center of human FLT3.The immunogen is located within amino acids 340 - 390 of FLT3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fms Related Tyrosine Kinase 3,Cd135,Flk-2,Flk2,Stk1,Flt3

Background

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]

Database

DATA IMAGES
GTX31863_3010_WB_20180221_18121410_734.jpg

GTX31863 WB Image

WB analysis of 3T3 cell lysate using GTX31863 FLT3 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX31863_1503_IHC-P_20180221_18121410_517.jpg

GTX31863 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31863 FLT3 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329