GeneTex
United States (US)

FMO3 antibody [N2C2], Internal

GTX103521_39897_20150410_WB_19011506_546.jpg
GTX103521_39897_WB_M_Liver_19011506_971.jpg
GTX103521_39897_IHC_19011506_115.jpg

Cat No. GTX103521

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A549 , H1299 , Mouse liver

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FMO3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Flavin Containing Monooxygenase 3,Fmoii,Tmau,Dj127D3.1,Fmo3

Cellular Localization

Microsome membrane , Endoplasmic reticulum membrane

Background

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Database

DATA IMAGES
GTX103521_39897_20150410_WB_19011506_546.jpg

GTX103521 WB Image

FMO3 antibody detects FMO3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FMO3 antibody (GTX103521) diluted at a dilution of 1:500.

GTX103521_39897_WB_M_Liver_19011506_971.jpg

GTX103521 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX103521 diluted at 1:500

GTX103521_39897_IHC_19011506_115.jpg

GTX103521 IHC-P Image

FMO3 antibody [N2C2], Internal detects FMO3 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
FMO3 antibody [N2C2], Internal (GTX103521) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169