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FMO3 antibody [N2C2], Internal

Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521
Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521
Anti-FMO3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103521

Cat No. GTX103521

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A549 , H1299 , Mouse liver

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FMO3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950296

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

flavin containing monooxygenase 3 , FMOII , TMAU , dJ127D3.1

Cellular Localization

Microsome membrane , Endoplasmic reticulum membrane

Background

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521

GTX103521 WB Image

FMO3 antibody detects FMO3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FMO3 antibody (GTX103521) diluted at a dilution of 1:500.

Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521

GTX103521 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX103521 diluted at 1:500

Anti-FMO3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103521

GTX103521 IHC-P Image

FMO3 antibody [N2C2], Internal detects FMO3 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
FMO3 antibody [N2C2], Internal (GTX103521) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for FMO3 antibody [N2C2], Internal (GTX103521). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FMO3 antibody [N2C2], Internal (GTX103521). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169