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FMO3 antibody [N2C2], Internal

Anti-FMO3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103521
Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521
Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521

Cat. No. GTX103521

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FMO3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Mouse liver , A549 , H1299

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FMO3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950296

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flavin containing monooxygenase 3 , FMOII , TMAU , dJ127D3.1

Cellular Localization

Microsome membrane , Endoplasmic reticulum membrane

Background

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FMO3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103521

GTX103521 IHC-P Image

FMO3 antibody [N2C2], Internal detects FMO3 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
FMO3 antibody [N2C2], Internal (GTX103521) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521

GTX103521 WB Image

FMO3 antibody detects FMO3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FMO3 antibody (GTX103521) diluted at a dilution of 1:500.

Anti-FMO3 antibody [N2C2], Internal used in Western Blot (WB). GTX103521

GTX103521 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX103521 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169