*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , H1299 , Mouse liver
Rat, Rabbit, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human FMO3. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Flavin Containing Monooxygenase 3,Fmoii,Tmau,Dj127D3.1,Fmo3
Microsome membrane , Endoplasmic reticulum membrane
The mammalian flavin-containing monooxygenases (FMO; EC 18.104.22.168) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]
GTX103521 WB Image
FMO3 antibody detects FMO3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FMO3 antibody (GTX103521) diluted at a dilution of 1:500.
GTX103521 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX103521 diluted at 1:500
GTX103521 IHC-P Image
FMO3 antibody [N2C2], Internal detects FMO3 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
FMO3 antibody [N2C2], Internal (GTX103521) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min