APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88748-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RTGKDRNQKKEKPD, from the internal region (near the C Terminus) of the protein sequence according to NP_002015.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fragile X mental retardation 1 , FMRP , FRAXA , POF , POF1
Cellular Localization
Nucleus , Cytoplasm , Cell projection, dendrite , Cell junction, synapse , Cell membrane , Nucleolus
Background
The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
|
GTX88748 IHC-P Image
IHC-P analysis of human placenta using GTX88748 FMRP antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX88748 WB Image
WB analysis of Kelly cell lysate using GTX88748 FMRP antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for FMRP antibody, Internal (GTX88748). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FMRP antibody, Internal (GTX88748). Be the first to share your experience with this product.
