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FMRP antibody

Anti-FMRP antibody used in Western Blot (WB). GTX134725
Anti-FMRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134725

Cat. No. GTX134725

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all FMRP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Predict Reactivity

Human, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse FMRP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887333

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fragile X mental retardation 1 , FMRP , Fmr-1

Database

Research Area

DATA IMAGES

Anti-FMRP antibody used in Western Blot (WB). GTX134725

GTX134725 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FMRP antibody (GTX134725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FMRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134725

GTX134725 ICC/IF Image

FMRP antibody detects FMRP protein at cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FMRP stained by FMRP antibody (GTX134725) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169