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GeneTex
United States (US)

FN3K antibody [N1C3-2]

GTX107579_40415_WB_19011506_322.jpg
GTX107579_40415_IHC_19011506_765.jpg

Cat No. GTX107579

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
See all FN3K products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FN3K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fructosamine 3 Kinase,Fn3K

Background

FN3K catalyzes phosphorylation of fructosamines formed by glycation, the nonenzymatic reaction of glucose with primary amines followed by Amadori rearrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and result in deglycation of glycated proteins (Delpierre et al., 2000 [PubMed 11016445]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX107579_40415_WB_19011506_322.jpg

GTX107579 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX107579 diluted at 1:1000

GTX107579_40415_IHC_19011506_765.jpg

GTX107579 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using FN3K(GTX107579) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169