APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Jurkat (10 μM Etoposide treatment for 24hr) , Lovo , Lovo (200 ng/ml EGF treatment for 0.5 hr)
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.47 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human FNBP1L. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
formin binding protein 1 like , C1orf39 , TOCA1
Cellular Localization
Cytoplasm , cytoskeleton , cell cortex , Cytoplasmic vesicle , Cell membrane; Peripheral membrane protein; Cytoplasmic side
Background
The protein encoded by this gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX132563 WB Image
Untreated (–) and treated (+) LoVo whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FNBP1L antibody (GTX132563) diluted at 1:1000.
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GTX132563 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FNBP1L antibody (GTX132563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for FNBP1L antibody (GTX132563). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FNBP1L antibody (GTX132563). Be the first to share your experience with this product.
