APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , GTX85425
Product Note
This antibody is specific for TOCA-1 C-Terminus
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TOCA-1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human TOCA-1.The immunogen is located within the last 50 amino acids of TOCA-1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
formin binding protein 1 like , C1orf39 , TOCA1
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton,Cytoplasmic vesicle,Cell membrane
Background
Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
Database
Research Area
DATA IMAGES
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GTX85425 WB Image
WB analysis of mouse brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85425 FNBP1L antibody.
Working concentration : 1 μg/ml
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GTX85425 IHC-P Image
IHC-P analysis of human brain tissue using GTX85425 FNBP1L antibody.
Working concentration : 20 μg/ml
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GTX85425 IHC-P Image
IHC-P analysis of human brain tissue using GTX85425 FNBP1L antibody.
Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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