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FOXA1 antibody [3C1]

Anti-FOXA1 antibody [3C1] used in Western Blot (WB). GTX84486
Anti-FOXA1 antibody [3C1] used in Western Blot (WB). GTX84486

Cat No. GTX84486

Host

Mouse

Clonality

Monoclonal

Clone Name

3C1

Isotype

IgG2b

Application

WB

Reactivity

Human, Dog
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human FOXA1 (NP_004487) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

forkhead box A1 , HNF3A , TCF3A

Cellular Localization

Nucleus

Background

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate | FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription | required for ESR1 binding to the NKX2-1 promoter in breast cancer cells | binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Database

Research Area

DATA IMAGES

Anti-FOXA1 antibody [3C1] used in Western Blot (WB). GTX84486

GTX84486 WB Image

WB analysis of various cell lines using GTX84486 FOXA1 antibody [3C1].
Loading : 35 ug per lane

Anti-FOXA1 antibody [3C1] used in Western Blot (WB). GTX84486

GTX84486 WB Image

WB analysis of HEK293T cells transfected with FOXA1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84486 FOXA1 antibody [3C1].
Loading : 5 ug per lane

REFERENCE

There are currently no references for FOXA1 antibody [3C1] (GTX84486). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FOXA1 antibody [3C1] (GTX84486). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf