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FOXA1 antibody [FOXA1/1515]

Anti-FOXA1 antibody [FOXA1/1515] used in Western Blot (WB). GTX34738
Anti-FOXA1 antibody [FOXA1/1515] used in IHC (Paraffin sections) (IHC-P). GTX34738
Anti-FOXA1 antibody [FOXA1/1515] used in IHC (Paraffin sections) (IHC-P). GTX34738
Anti-FOXA1 antibody [FOXA1/1515] used in Flow cytometry (FACS). GTX34738

Cat. No. GTX34738

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 FOXA1/1515

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all FOXA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 0.5-1μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human FOXA1 protein fragment (aa372-472) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FOXA1 , HNF3A , TCF3A , forkhead box A1

Cellular Localization

Nucleus

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXA1 antibody [FOXA1/1515] used in Western Blot (WB). GTX34738

GTX34738 WB Image

WB analysis of HeLa cell lysate using GTX34738 FOXA1 antibody [FOXA1/1515].

Anti-FOXA1 antibody [FOXA1/1515] used in IHC (Paraffin sections) (IHC-P). GTX34738

GTX34738 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34738 FOXA1 antibody [FOXA1/1515].

Anti-FOXA1 antibody [FOXA1/1515] used in IHC (Paraffin sections) (IHC-P). GTX34738

GTX34738 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34738 FOXA1 antibody [FOXA1/1515].

Anti-FOXA1 antibody [FOXA1/1515] used in Flow cytometry (FACS). GTX34738

GTX34738 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX34738 FOXA1 antibody [FOXA1/1515].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529